Journal: Science Advances
Article Title: Cancer-associated SF3B1-K700E mutation controls immune responses by regulating T reg function via aberrant Anapc13 splicing
doi: 10.1126/sciadv.ado4274
Figure Lengend Snippet: ( A ) Body weight of indicated 8- to 10-week-old or 30- to 35-week-old mice ( n ≥ 5 per genotype per group). ( B ) Image of spleens from indicated 8- to 10-week-old (left) or 30- to 35-week-old mice (right). ( C ) Weight of the spleens from indicated 8- to 10-week-old or 30- to 35-week-old mice ( n ≥ 4 per genotype per group). ( D and E ) Total number of cells (D) and CD3 + , CD4 + , and CD8 + T cells [(E), left] of spleens or inguinal lymph nodes (iLN) or mLNs [(E), right] from indicated mice ( n ≥ 4 per genotype per group). ( F ) Percentage of CD44 hi CD62 lo memory-like (left) and CD44 lo CD62 hi (right) naive cells among splenic CD4 + T cells from indicated aged mice ( n ≥ 4 per genotype per group). ( G ) Representative flow cytometric analysis (left) and percentage (right) of IL-17A + and IFN-γ + cells among CD4 + T cells from spleens or mLN of indicated aged mice ( n ≥ 4 per genotype). ( H ) Section of H&E-stained lung and liver from indicated 30- to 35-week-old mice. ( I ) Representative flow cytometric analysis (left) and percentage (right) of T regs (Foxp3 + ) among CD4 + T cells recovered from lung and liver of 30- to 35-week-old mice ( n ≥ 4 per genotype). Boxed area: cell population of interest. Data are from three experiments [(A) and (C) to (F); (G) and (I), right panels, presented as means ± SEM] or are from one representative of three independent experiments [(B) and (H); (G) and (I), left panels].* P < 0.05, ** P < 0.01, and *** P < 0.001 (two-tailed Student’s t test).
Article Snippet: Monoclonal antibodies against mouse CD3 (145-2C11), CD28 (37.51), IL-4 (11B11), and IFN-γ (XMG1.2), as well as phycoerythrin (PE)-conjugated anti-CD8 (dilution ratio, 1:100; 53-6.7), allophycocyanin-conjugated anti-Foxp3 (dilution ratio, 1:100; FJK-16s), PE-indotricarbocyanine (Cy7)–conjugated anti–IL-17A (dilution ratio, 1:100; eBio17B7), APC-conjugated anti–IFN-γ (dilution ratio, 1:100; XMG1.2), and Live/Dead Fixable Near-IR Dead Cell Stain (dilution ratio, 1:1000; L34976) were from Invitrogen.
Techniques: Staining, Two Tailed Test
Bio-Rad is a verified supplier 
![( A ) Body weight of indicated 8- to 10-week-old or 30- to 35-week-old mice ( n ≥ 5 per genotype per group). ( B ) Image of spleens from indicated 8- to 10-week-old (left) or 30- to 35-week-old mice (right). ( C ) Weight of the spleens from indicated 8- to 10-week-old or 30- to 35-week-old mice ( n ≥ 4 per genotype per group). ( D and E ) Total number of cells (D) and CD3 + , CD4 + , and <t>CD8</t> + T cells [(E), left] of spleens or inguinal lymph nodes (iLN) or mLNs [(E), right] from indicated mice ( n ≥ 4 per genotype per group). ( F ) Percentage of CD44 hi CD62 lo memory-like (left) and CD44 lo CD62 hi (right) naive cells among splenic CD4 + T cells from indicated aged mice ( n ≥ 4 per genotype per group). ( G ) Representative flow cytometric analysis (left) and percentage (right) of IL-17A + and IFN-γ + cells among CD4 + T cells from spleens or mLN of indicated aged mice ( n ≥ 4 per genotype). ( H ) Section of H&E-stained lung and liver from indicated 30- to 35-week-old mice. ( I ) Representative flow cytometric analysis (left) and percentage (right) of T regs (Foxp3 + ) among CD4 + T cells recovered from lung and liver of 30- to 35-week-old mice ( n ≥ 4 per genotype). Boxed area: cell population of interest. Data are from three experiments [(A) and (C) to (F); (G) and (I), right panels, presented as means ± SEM] or are from one representative of three independent experiments [(B) and (H); (G) and (I), left panels].* P < 0.05, ** P < 0.01, and *** P < 0.001 (two-tailed Student’s t test).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4738/pmc11414738/pmc11414738__sciadv.ado4274-f4.jpg)